Evidence that toxicity of lipopolysaccharide upon cholinergic basal forebrain neurons requires the presence of glial cells in vitro

The cholinergic system of the basal forebrain is affected in brains of dementia patients and during neuroinflammation. The aim of this study was to establish a method to cultivate basal forebrain cholinergic neurons in dissociated, pure neuronal cultures and to apply this method to study the effect...

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Bibliographic Details
Published in:Brain research bulletin 2002-05, Vol.58 (1), p.91-98
Main Authors: Weis, Carla, Humpel, Christian
Format: Article
Language:English
Subjects:
Age Factors
Animals
Biological and medical sciences
Cell Communication - drug effects
Cell Count
Cell Survival - drug effects
Cells, Cultured
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Choline acetyltransferase
Choline O-Acetyltransferase - analysis
Cholinergic Fibers - drug effects
Fundamental and applied biological sciences. Psychology
Histocompatibility Antigens Class I - analysis
Histocompatibility Antigens Class II - analysis
Immunocytochemistry
Immunohistochemistry
In Vitro Techniques
Lipopolysaccharides - toxicity
Microtubule-associated protein-2
Microtubule-Associated Proteins - analysis
Neuroglia - cytology
Neurons - drug effects
Neurons - ultrastructure
p75 Receptor
Prosencephalon - cytology
Rats
Rats, Sprague-Dawley
Receptor, Nerve Growth Factor
Receptors, Nerve Growth Factor - analysis
Sandwich-technique
Vertebrates: nervous system and sense organs
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Summary:The cholinergic system of the basal forebrain is affected in brains of dementia patients and during neuroinflammation. The aim of this study was to establish a method to cultivate basal forebrain cholinergic neurons in dissociated, pure neuronal cultures and to apply this method to study the effect of acute and chronic experimentally-induced inflammation using lipopolysaccharide. Purity of the cultures, degrees of neuronal dissociation, connectivity and neuronal survival were investigated by immunocytochemistry for microtubule-associated protein-2 (neurons), glial fibrillary acidic protein (astroglia), complement receptor 3 (microglia), choline acetyltransferase and the neurotrophin receptor p75 (cholinergic neurons). Neuronal cultures only contained
ISSN:0361-9230
1873-2747
DOI:10.1016/S0361-9230(02)00764-5