Advances in cooled semen technologies: seminal plasma and semen extender

• Published in: Animal reproduction science 2001-12, Vol.68 (3), p.171-180
• Main Authors: Rigby, S.L, Brinsko, S.P, Cochran, M, Blanchard, T.L, Love, C.C, Varner, D.D
• Format: Article
• Language: English
• Subjects: Animals; Cryopreservation - methods; Cryopreservation - veterinary; Equine; Female; Fertility; Horses - physiology; Insemination, Artificial - veterinary; Male; Pregnancy; Random Allocation; Semen; Semen extender; Semen Preservation - methods; Semen Preservation - veterinary; Seminal plasma; Sperm Motility - physiology; Spermatozoa
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/S0378-4320(01)00154-3

This study evaluated motility and fertility of uncentrifuged and centrifuged equine semen following dilution in a skim milk-glucose extender with or without supplemental Tyrode’s medium. In addition, the effect of seminal plasma addition to each extender was evaluated. For Experiment 1, motility of 48 h cooled, stored spermatozoa was evaluated following eight dilution treatments: uncentrifuged and diluted 1:4 (v/v) in skim milk-glucose extender (EZ Mixin CSTJ; CST-1:4) or in CST supplemented 65:35 (v/v) with modified Tyrode’s medium (KMT-1:4); uncentrifuged and diluted to 25×10 6 spermatozoa/ml in CST (CST-1:9) or in KMT (KMT-1:9); centrifuged and diluted in CST with 0% seminal plasma (CST-0) or 20% seminal plasma (CST-20) or centrifuged and diluted in KMT containing 0% seminal plasma (KMT-0) or in KMT containing 20% seminal plasma (KMT-20). Sperm motility parameters evaluated included percentage of total motile sperm (% TMOT), percentage of progressively motile sperm (% PMOT), curvilinear velocity (VCL) and straight-line velocity (VSL). Mean % PMOT was lower ( P