Determination of serotonin, its precursors, metabolites and [ 3H]serotonin in lung by high-performance liquid chromatography with fluorescence detection

A rapid, simple, sensitive method for the determination of serotonin, its precursors, metabolites and [ 3H]serotonin in lung is described. Tissue preparation requires only homogenization in dilute perchloric acid and centrifugation prior to separation by high-performance liquid chromatography using...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1981-09, Vol.225 (1), p.27-35
Main Authors: Mais, Dale E., Lahr, Patricia D., Bosin, Talmage R.
Format: Article
Language:English
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Summary:A rapid, simple, sensitive method for the determination of serotonin, its precursors, metabolites and [ 3H]serotonin in lung is described. Tissue preparation requires only homogenization in dilute perchloric acid and centrifugation prior to separation by high-performance liquid chromatography using a reversed-phase column. Detection is based on the native fluorescence of indole compounds and detection limits ranged between 30–90 pg injected. The method has been used to determine these compounds in mouse lung and plasma.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)80240-6