Determination of bucindolol and its major metabolites in plasma by capillary gas chromatography—mass spectrometry

A method for determining bucindolol, a deuterated analogue and two metabolites in plasma samples is described. Analytes are obtained from plasma by liquid extraction of 1 ml of plasma at pH 10.5 with 7 ml of peroxide-free ether—hexane (4:1). Following derivatization with 50 μl of N-methyl-N-trimethy...

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Bibliographic Details
Published in:Journal of chromatography 1986, Vol.377, p.183-194
Main Authors: Bartek, M.J., Kerns, E.H., Gammans, R.E., Gallo, D.G.
Format: Article
Language:English
Subjects:
Analysis
Applied sciences
Biological and medical sciences
Exact sciences and technology
General pharmacology
Medical sciences
Other techniques and industries
Pharmacology. Drug treatments
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Description
Summary:A method for determining bucindolol, a deuterated analogue and two metabolites in plasma samples is described. Analytes are obtained from plasma by liquid extraction of 1 ml of plasma at pH 10.5 with 7 ml of peroxide-free ether—hexane (4:1). Following derivatization with 50 μl of N-methyl-N-trimethylsilyl trifluoroacetamide—pyridine (1:1) for 20 min at 65°C the samples are analyzed by capillary column gas chromatography—mass spectrometry with selected-ion monitoring. Quantification is linear over the range 2–500 ng/ml. Single-day precision was typically 6.2% or better for bucindolol and 13% or better for the metabolites.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)80773-2