High-performance liquid chromatographic separation and isolation of quinidine and quinine metabolites in rat urine

A procedure for the separation and isolation of the urinary metabolites of quinidine and quinine by reversed-phase high-performance liquid chromatography is described. Nine metabolites of quinidine and eight metabolites of quinine were detected in the urine of male Sprague-Dawley rats after a single...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1980-02, Vol.181 (2), p.219-226
Main Authors: Barrow, Susan E., Taylor, A.A., Horning, E.C., Horning, M.G.
Format: Article
Language:English
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Summary:A procedure for the separation and isolation of the urinary metabolites of quinidine and quinine by reversed-phase high-performance liquid chromatography is described. Nine metabolites of quinidine and eight metabolites of quinine were detected in the urine of male Sprague-Dawley rats after a single dose of quinidine or quinine (50 mg kg −1). Following extraction from urine, the metabolites were separated on either an analytical or a semi-preparative reversed-phase column by gradient elution. After isolation and derivatization, the metabolites were analyzed by gas chromatography and gas chromatography—mass spectrometry.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)81607-2