High-performance ion-exchange chromatography of myosin using a DEAE-5PW column

High-performance ion-exchange chromatography of myosin using a DEAE-5PW packing was used to purify myosin from skeletal, cardiac and smooth muscle. This method produces high-speed resolution (30-min analysis) of myosin from contaminating myofibrillar proteins. The column has a high capacity for bind...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1989, Vol.491 (1), p.61-68
Main Authors: Lema, Mark J., Pluskal, Malcolm G., Allen, Paul D.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Contractile proteins
Fundamental and applied biological sciences. Psychology
Holoproteins
Proteins
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High-performance ion-exchange chromatography of myosin using a DEAE-5PW packing was used to purify myosin from skeletal, cardiac and smooth muscle. This method produces high-speed resolution (30-min analysis) of myosin from contaminating myofibrillar proteins. The column has a high capacity for binding myosin (up to 1 g) and can be used for small-scale preparation of highly purified myosin. Gel analysis in the presence of sodium dodecyl sulfate showed recovery of myosin with very little contamination of other myofibrillar proteins. Myosin was also recovered from small biopsy samples (0.1 g) by a direct extraction technique with recovery of biological ATPase activity.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)82819-4