Measurement of β-carbolines by high-performance liquid chromatography with fluorescence detection

A method using high-performance liquid chromatography with fluorescence detection was developed for the determination of β-carboline compounds norharman, harman, norharmol, and harmol in lung. Aqueous derivatization with acetic anhydride was used to facilitate the isolation and separation of the phe...

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Bibliographic Details
Published in:Journal of chromatography. Biomedical applications 1988, Vol.428, p.229-236
Main Authors: Bosin, Talmage R., Faull, Kym F.
Format: Article
Language:English
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Summary:A method using high-performance liquid chromatography with fluorescence detection was developed for the determination of β-carboline compounds norharman, harman, norharmol, and harmol in lung. Aqueous derivatization with acetic anhydride was used to facilitate the isolation and separation of the phenolic compounds and to reduce the fluorescence background of the biological samples. Harman was identified and quantitated in rat lung (1.88 ± 0.55 ng/g) using this method and its identity confirmed by means of gas chromatography—negative-ion chemical ionization mass spectrometry.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)83913-4