Capillary gas chromatographic profiling of total long-chain fatty acids cholesterol in biological materials

The profiling of total long-chain fatty acids and cholesterol in a variety of biological materials, using capillary gas chromatography with flame ionization detection, is described. The within-run precision and day-to-dy precision for fifteen fatty acids and cholesterol in erythrocyte samples were i...

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Bibliographic Details
Published in:Journal of chromatography 1983-01, Vol.278 (2), p.231-244
Main Authors: Muskiet, Frits A.J., van Doormaal, Jasper J., Martini, Ingrid A., Wolthers, Bert G., van der Slik, Wim
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Lipids
Other biological molecules
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Summary:The profiling of total long-chain fatty acids and cholesterol in a variety of biological materials, using capillary gas chromatography with flame ionization detection, is described. The within-run precision and day-to-dy precision for fifteen fatty acids and cholesterol in erythrocyte samples were investigated. Quantitative data on the analysis of amniotic fluid samples collected from the women in the 30th week are given together with a correlation study on their lecithin/sphingomyelin and their palmitic acid/stearic ratios. In addition, the method was applied to lumbar cerebrospinal fluid, plasma, isolated leukemia blood cells and neuroblastoma tissue.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)84782-9