Determination of a new antibacterial agent (at-2266) and its metabolites in plasma and urine by high-performance liquid chromatography

• Published in: Journal of chromatography 1983-01, Vol.278 (2), p.321-328
• Main Authors: Nakamura, Reiko, Yamaguchi, Toshikazu, Sekine, Yutaka, Hashimoto, Masahisa
• Format: Article
• Language: English
• Subjects: Analysis; Biological and medical sciences; General pharmacology; Medical sciences; Pharmacology. Drug treatments
• ISSN: 0378-4347; 0021-9673
• DOI: 10.1016/S0378-4347(00)84791-X

A high-performance liquid chromatographic method has been developed which enables accurate determination of a new synthetic antibacterial agent, AT-2266, and its metabolite, M-2, in plasma, and AT-2266 and its five metabolites, M-1, M-2, M-3, M-4 and M-5, in urine. AT-2266 is extracted as ethyl carbamate with chloroform containing 1% ethyl chloroformate and assayed on a liquid chromatograph equipped with an ultraviolet detector at 340 nm. Accurate determinations are possible over a concentration range of 0.1–10 μ/ml AT-2266 in plasma, and 1–500 μ/ml AT-2266 in urine. The coefficient of variation at the 2 μ/ml level of AT-2266 is 1.9% (n  6). The minimum detectable concentrations of AT-2266 in plasma and urine are 0.01 μ/ml and 0.1 μ/ml, respectively, and those of other metabolites are similar to those of AT-2266. Plasma levels and urinary excretion of AT-2266 in a man following single oral administration (400 mg) have also been determined.