Identification and quantitation of a metabolite of anethol dithiolthione in rat and mouse urine using high-performance liquid chromatography

Urine samples from rats and mice fed anethol dithiolthione (ADT [3-( pmethoxyphenyl)-1,2-dithiol-3-thione] were analyzed using reversed-phase high-performance liquid chromatogrphy. Urine was introduced directly on the liquid chromatograph which was modified by replacing the sample loop with a guard...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography 1983-01, Vol.276 (1), p.111-119
Main Authors: Masoud, Asaad N., Bueding, Ernest
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Urine samples from rats and mice fed anethol dithiolthione (ADT [3-( pmethoxyphenyl)-1,2-dithiol-3-thione] were analyzed using reversed-phase high-performance liquid chromatogrphy. Urine was introduced directly on the liquid chromatograph which was modified by replacing the sample loop with a guard column. Highly polar urine components were washed off the guard column prior to chromatography. A major metabolite and the parent compound (ADT) were separated and detected using the chromatographic conditions described in this study. The metabolite was identified as desmethyl ADT. The identification was based on co-chromatography on two columns using two mobile phases and peak height ratios of the metabolite and the references standard. Data pertaining to the pattern of excretion of ADT and desmethyl ADT in the animals studied are reported.
ISSN:0378-4347
0021-9673
DOI:10.1016/S0378-4347(00)85071-9