Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China

• Published in: Journal of ethnopharmacology 2000-09, Vol.72 (1), p.239-246
• Main Authors: Banskota, Arjun H, Tezuka, Yasuhiro, Adnyana, I Ketut, Midorikawa, Kiyoshi, Matsushige, Katsumichi, Message, Dejair, Huertas, Alfredo A.G, Kadota, Shigetoshi
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - pharmacology; Bepridil - analogs & derivatives; Bepridil - chemistry; Biological and medical sciences; Biphenyl Compounds; Brazil; Cell Death - drug effects; Chemical and Drug Induced Liver Injury - prevention & control; China; Cytotoxicity; DPPH free radical scavenging activity; Drug Screening Assays, Antitumor; Free Radical Scavengers - pharmacology; Free Radicals; General pharmacology; Hepatocytes - drug effects; Hepatoprotective activity; Humans; Medical sciences; Mice; Netherlands; Peru; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Picrates; Propolis; Propolis - pharmacology; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/S0378-8741(00)00252-X

Propolis is a resinous hive product collected by honeybees from various plant sources. The composition of the propolis depends upon the time, vegetation and the area of collection. Thus, quality evaluation of the propolis is important, before use in food and beverages. For this propose three different biological activities were carried out, i.e. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, cytotoxicity and hepatoprotective activity, of MeOH and water extracts of nine different propolis from Brazil, Peru, the Netherlands and China. The results showed that water extracts of six Brazilian and a Chinese propolis possessed stronger DPPH free radical scavenging activity than the corresponding MeOH extract, whereas in the case of Netherlands and Peruvian propolis MeOH extract exhibited stronger DPPH free radical scavenging activity. The MeOH extracts of all propolis possessed stronger cytotoxicity than the corresponding water extract towards murine colon 26-L5 carcinoma and human HT-1080 fibrosarcoma cells. The result of hepatoprotective activity of Brazilian propolis on d-galactosamine ( d-GalN)/tumor necrosis factor-α (TNF-α)-induced cell death in primary cultured mouse hepatocytes were found in accordance with the grade set up by beekeepers in Brazil.