Significance of sulfate- O-3-Xyl-MU synthesized by cultured human skin fibroblasts

• Published in: International Congress series 2001-12, Vol.1223, p.199-203
• Main Authors: Tazawa, Toshiyuki, Matsuya, Hideki, Kudo, Daisuke, Ishido, Keinosuke, Yoshihara, Syuichi, Sasaki, Mutsuo
• Format: Article
• Language: English
• Subjects: 4-Methylumbelliferyl-β- d-xyloside; Glycosaminoglycan biosynthesis; Sulfate- O-3-xylosylβ1-(4-methylumbelliferone)
• ISSN: 0531-5131; 1873-6157
• DOI: 10.1016/S0531-5131(01)00423-X

Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-β- d-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xyl-MU derivatives. This Xyl-MU derivative was subjected to structural analysis and the results indicated that it was sulfate- O-3-Xyl-MU. This Xyl-MU derivative was also synthesized when Xyl-MU was incubated with [ 35S]PAPS. But when Gal-Xyl-MU and Gal-Gal-Xyl-MU were incubated with [ 35S]PAPS, incorporation of [ 35S]sulfate from [ 35S]PAPS into either galactose or xylose was not observed. When Xyl-MU or sulfate-Xyl-MU was incubated with UDP-[ 3H]Gal, incorporation of [ 3H]Gal into Xyl-MU was observed, but incorporation into sulfate-Xyl-MU was not. These results indicate that chain elongation from Xyl-MU is inhibited by sulfation of Xyl-MU, and that Xyl-MU sulfation is involved in the control of Xyl-MU-initiated glycosaminoglycan biosynthesis.