Determination of cobalamins using capillary electrophoresis inductively coupled plasma mass spectrometry

• Published in: Spectrochimica acta. Part B: Atomic spectroscopy 2000-12, Vol.55 (12), p.1823-1832
• Main Authors: Baker, S.A, Miller-Ihli, N.J
• Format: Article
• Language: English
• Subjects: Capillary electrophoresis; Cobalamins; ICP-MS; Micellar electrokinetic chromatography; Vitamin B12
• ISSN: 0584-8547; 1873-3565
• DOI: 10.1016/S0584-8547(00)00271-8

The determination of cobalamins using capillary electrophoresis inductively coupled plasma mass spectrometry (CE-ICP-MS) was investigated. Both capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) modes of operation were studied. The optimal separation of four cobalamin species (cyanocobalamin, hydroxocobalamin, methylcobalamin, and 5′-deoxyadenosylcobalamin) and a potentially harmful corrinoid analogue (cobinamide dicyanide) was obtained using CZE at a pH of 2.5. Both 20 mM phosphate and 20 mM formate buffers were used with success, although the formate buffer provided improved resolution. The CZE-ICP-MS method was used to quantify cyanocobalamin in a vitamin supplement and the analytical results were in good agreement (±5%) with values obtained by ICP-MS for total Co levels. The solution detection limits for cobalamins using CZE-ICP-MS were approximately 50 ng/ml. MEKC was found to be useful for the screening of vitamin preparations because it provided a rapid means of distinguishing cyanocobalamin (the form most commonly used in vitamin preparations) from free cobalt. The separation of free cobalt and cyanocobalamin using MEKC was achieved in less than 10 min.