Computerized analysis of sperm motion: Effects of glycerol concentration on the cryopreservation of equine spermatozoa

• Published in: Journal of equine veterinary science 1995-09, Vol.15 (9), p.377-380
• Main Authors: Burns, P.J., Reasner, D.S.
• Format: Article
• Language: English
• ISSN: 0737-0806; 1542-7412
• DOI: 10.1016/S0737-0806(07)80480-1

An experiment was conducted to examine the effects of glycerol added to a glucose-sucrose-dried skim milk freezing extender (B4) at 0, 1, 2, or 4% for cryopreservation of equine spermatozoa. Two ejaculates from each of three stallions were used in the study. Following cryopreservation and storage in liquid nitrogen for at least 30 days, the semen was thawed and subsequently diluted to approximately 33 × 10 6 sperm/ml in the freezing extender minus glycerol and egg yolk at 30°C. It was then incubated for 1 h at room temperature prior to evaluation. Each semen sample was evaluated for the percentage of motile sperm (M, %), the percentage progressively motile sperm (PM, %), velocity (V, μ/sec), progressive velocity (PV, μ/sec), linearity (L, %) and lateral head displacement (LHD, μ) using computer-aided sperm analysis (CASA). The data were statistically analyzed and the optimal glycerol concentration was determined for each parameter. For three of the parameters (M, PM and LHD), the 2% level of glycerol was selected by the statistical analysis as optimal (P