Induction of micronuclei formation in preimplantation mouse embryos after maternal treatment with 2-bromopropane

We examined effects of 2-bromopropane (2-BP), a chlorofluorocarbon replacement, on mouse embryonic mutagenicity. 2-BP was administered to pregnant mice intraperitoneally (i.p.) (300, 600, 900, and 1800 mg/kg) during the early preimplantation period. On day 3 of gestation, micronuclei (MN) frequency...

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Bibliographic Details
Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2000, Vol.15 (1), p.81-85
Main Authors: Ishikawa, Hitoshi, Tian, Ying, Yamauchi, Toru
Format: Article
Language:English
Subjects:
2-Bromopropane
Cell number
Embryotoxicity
Mice
Micronuclei
Preimplantation
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Summary:We examined effects of 2-bromopropane (2-BP), a chlorofluorocarbon replacement, on mouse embryonic mutagenicity. 2-BP was administered to pregnant mice intraperitoneally (i.p.) (300, 600, 900, and 1800 mg/kg) during the early preimplantation period. On day 3 of gestation, micronuclei (MN) frequency and embryo cell number were determined. 2-BP induced a dose-related significant increase in MN frequency and a treatment-related decrease in embryo cell number. Furthermore, the cell numbers were significantly smaller in the MN-positive embryos by two-way ANOVA taking it into account an interactive effect between 2-BP dose and the presence or absence of MN. A simultaneous decrease in cell number and increase in MN frequency may reflect an embryonic developmental disadvantage resulting from maternal treatment with 2-BP. Further study is needed to establish how 2-BP contributes to postimplantation embryonic development.
ISSN:0890-6238
1873-1708
DOI:10.1016/S0890-6238(00)00112-X