Apolipoprotein E Stimulates Bone Formation on Titanium in Vitro

Objective: To examine the effects of apolipoprotein E2, a strongly expressed molecule during titanium implant healing, on bone formation by rat primary osteoblasts over titanium. Materials and Methods: Primary rat osteoblasts were cultured on titanium in the presence of 0.011.0 μg/mL apolipoprotein...

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Bibliographic Details
Published in:The Asian journal of oral and maxillofacial surgery 2007-06, Vol.19 (2), p.96-100
Main Authors: Kodama, Takashi, Goto, Tetsuya, Ishibe, Toru, Kobayashi, Shigeru, Takahashi, Tetsu
Format: Article
Language:English
Subjects:
Apolipoproteins
Bone and bones
Osteoblasts
Osteocalcin
Titanium
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Summary:Objective: To examine the effects of apolipoprotein E2, a strongly expressed molecule during titanium implant healing, on bone formation by rat primary osteoblasts over titanium. Materials and Methods: Primary rat osteoblasts were cultured on titanium in the presence of 0.011.0 μg/mL apolipoprotein E2. After 2 or 3 weeks of culture, bone formation was analysed by staining the bone-like nodules with calcein blue. The mRNAs of the osteogenic genes type I collagen, osteopontin, and osteocalcin, were analysed using semi-quantitative reverse transcriptase polymerase chain reaction. Results: Apolipoprotein E2 enhanced bone-like nodule formation on titanium in a dose-dependent manner. Apolipoprotein E2 strongly upregulated osteopontin and osteocalcin mRNA expression, but had only a small effect on type I collagen mRNA expression. Conclusion: Apolipoprotein E2 stimulated bone formation on titanium disks in vitro. This may lead to a new approach to accelerating implant healing.
ISSN:0915-6992
2212-1897
DOI:10.1016/S0915-6992(07)80023-9