Evaluation of an indirect enzyme-linked immunoassay for presumptive serodiagnosis of Brucella ovis in sheep

An indirect enzyme-linked immunoassay (IELISA) for the detection of antibodies to Brucella ovis was evaluated. Relative to the complement fixation test (CFT) the sensitivity of the IELISA was 96.3% and the specificity was 99.6%. The sensitivity and specificity obtained in this study were comparable...

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Bibliographic Details
Published in:Small ruminant research 2003-06, Vol.48 (3), p.173-179
Main Authors: Gall, D, Nielsen, K, Vigliocco, A, Smith, P, Perez, B, Rojas, X, Robles, C
Format: Article
Language:English
Subjects:
antibodies
Brucella melitensis biovar Ovis
Brucella ovis
complement fixation tests
Enzyme-linked immunoassay
enzyme-linked immunosorbent assay
gels
IELISA
serodiagnosis
Sheep
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Summary:An indirect enzyme-linked immunoassay (IELISA) for the detection of antibodies to Brucella ovis was evaluated. Relative to the complement fixation test (CFT) the sensitivity of the IELISA was 96.3% and the specificity was 99.6%. The sensitivity and specificity obtained in this study were comparable to ELISAs and CFTs of other studies in which B. ovis isolation was used for evaluation making it a choice to replace current serological tests such as the agar gel immunodiffusion test and the CFT.
ISSN:0921-4488
1879-0941
DOI:10.1016/S0921-4488(03)00013-0