Caprine arthritis-encephalitis virus in semen of naturally infected bucks

Semen and peripheral blood mononuclear cells (PBMC) samples of 15 infected and 3 non-infected bucks were evaluated for the presence of caprine arthritis–encephalitis virus (CAEV), by double-nested polymerase chain reaction (d-n-PCR). In order to locat the presence of vı́rus, semen samples were separ...

Full description

Saved in:
Bibliographic Details
Published in:Small ruminant research 1999-04, Vol.32 (2), p.101-106
Main Authors: Travassos, C.E, Benoı̂t, C, Valas, S, da Silva, A.G, Perrin, G
Format: Article
Language:English
Subjects:
ADN
CAPRIN
CAPRINE ARTHRITIS ENCEPHALIT VIRUS
Caprine arthritis–encephalitis virus
CAPRINOS
DISEASE TRANSMISSION
DNA
GOATS
PCR
Polymerase chain reaction
SEMEN
SPERME
TRANSMISION DE ENFERMEDADES
Transmission
TRANSMISSION DES MALADIES
VIRUS ARTHRITE ENCEPHALITE CAPRINE
VIRUS ARTRITIS ENCEFALITIS CAPRINA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Semen and peripheral blood mononuclear cells (PBMC) samples of 15 infected and 3 non-infected bucks were evaluated for the presence of caprine arthritis–encephalitis virus (CAEV), by double-nested polymerase chain reaction (d-n-PCR). In order to locat the presence of vı́rus, semen samples were separated into cell-free seminal fluids (CFSF), enriched non-spermatozoal cells fraction (NSCF), and spermatozoa (SPZ). All PBMC samples from infected bucks were positive for CAEV-DNA but only 8 out of 15 semen samples were positive for CAEV-DNA. The presence of virus was not necessarily concordant in different fractions of semen from the same buck over time. In addition, the virus was isolated from CFSF in primary goat synovial membrane (GSM) cell culture, in which productive infection was demonstrated by syncytia formation. This is the first report describing the presence of CAEV-DNA and also of replication-competent virus in semen from CAEV seropositive bucks. Such results suggest the possibility of sexual transmission of CAEV.
ISSN:0921-4488
1879-0941
DOI:10.1016/S0921-4488(98)00172-2