Love wave acoustic immunosensor operating in liquid

A Love wave acoustic device has been utilized for monitoring antibody-antigen immunoreactions in aqueous solutions in real time. High sensitivity and selectivity are achieved. In this study, sheep IgG is immobilized on the device surface as a receptor layer for detection of antisheep IgG in buffer s...

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Bibliographic Details
Published in:Sensors and actuators. A. Physical. 1997-06, Vol.61 (1), p.279-286
Main Authors: Harding, G.L., Du, J., Dencher, P.R., Barnett, D., Howe, E.
Format: Article
Language:English
Subjects:
Acoustic sensors
Biosensors
IgG antibody
Immunosensors
Love waves
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Summary:A Love wave acoustic device has been utilized for monitoring antibody-antigen immunoreactions in aqueous solutions in real time. High sensitivity and selectivity are achieved. In this study, sheep IgG is immobilized on the device surface as a receptor layer for detection of antisheep IgG in buffer solution. The kinetics of the antibody-antigen binding are investigated by monitoring the frequency change against reaction time for a wide range of antibody concentrations. A general analytic expression for the frequency against time and antibody concentrations is derived. A response to a concentration of 1 ng ml −1 is unambiguously detected. A dual-channel delay-line configuration. with one channel for sensing and the other as reference, is used to give a comparison of specific and non-specific responses as well as to reduce the temperature effects. The Love wave sensor is robust, reliable and reusable many times.
ISSN:0924-4247
1873-3069
DOI:10.1016/S0924-4247(97)80275-0