pH-ISFET based urea biosensor

A new method of direct immobilization of urease on a hydrated silicon nitride surface with the use of glutaraldehyde is presented. The main parameters characterizing the developed urea biosensor based on pH-ion-selective field effect transistor (pH-ISFET) with directly immobilized urease are: (1) ma...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1997-10, Vol.44 (1), p.370-376
Main Authors: Pijanowska, Dorota G., Torbicz, Władysław
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Enzyme-modified field effect transistors (EnFET)
Fundamental and applied biological sciences. Psychology
Ion-selective field effect transistor (ISFET)
Methods. Procedures. Technologies
Urea biosensor
Urease
Various methods and equipments
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Summary:A new method of direct immobilization of urease on a hydrated silicon nitride surface with the use of glutaraldehyde is presented. The main parameters characterizing the developed urea biosensor based on pH-ion-selective field effect transistor (pH-ISFET) with directly immobilized urease are: (1) maximal analytical signal, 120–140 mV in 10 mM phosphate buffer solution; (2) linear range of Δ U gs= f(log C urea):p C urea(2–3.5) in 10 mM phosphate buffer; (3) response time, 80 s; and (4) lifetime of 35 days with a stable analytical signal. The biosensor was used for urea determination in blood serum and in hemodialysis fluid with satisfactory results.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(97)00194-9