Preparation and characterization of platinum coatings for long life-time BOD biosensor

An easy method to prepare platinum coatings is proposed. Platinum coatings were prepared on glass substrate by applying a thermal decomposition method. Electrochemical and surface properties of the Pt coatings obtained were studied. A polycrystalline Pt layer is formed which shows electrocatalytic a...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1998-01, Vol.47 (1), p.21-29
Main Authors: Tammeveski, Kaido, Kikas, Timo, Tenno, Toomas, Niinistö, Lauri
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biochemical oxygen demand (BOD) biosensor
Oxygen reduction
Oxygen sensor
Platinum coating
X-ray photoelectron spectroscopy
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Summary:An easy method to prepare platinum coatings is proposed. Platinum coatings were prepared on glass substrate by applying a thermal decomposition method. Electrochemical and surface properties of the Pt coatings obtained were studied. A polycrystalline Pt layer is formed which shows electrocatalytic activity towards oxygen reduction similar to that of bulk platinum. An amperometric oxygen sensor was constructed by using Pt-coated glass substrate as a cathode. The oxygen sensor exhibited good performance characteristics. A biosensor for the determination of biochemical oxygen demand (BOD) was developed using the oxygen sensor as a base transducer and by immobilizing bacteria Bacillus subtilis in agarose gel film on the top of the sensor's membrane. The BOD biosensor was tested in various media.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(98)00004-5