Interference-based amygdalin sensor with emulsin and peroxidase

An interference-based biosensor for amygdalin, a cyanogenic glycoside, is developed. Horseradish peroxidase (HRP) is adsorbed on a pyrolytic graphite (PG) electrode and then emulsin is cross-linked with glutaraldehyde on the electrode surface. The prepared bienzyme electrode is poised at −300 mV ver...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1998-07, Vol.49 (3), p.268-272
Main Authors: Tatsuma, Tetsu, Tani, Koichiro, Ogawa, Takashi, Oyama, Noboru
Format: Article
Language:English
Subjects:
Amygdalin
Cyanogenic glycoside
Emulsin
Enzyme electrode
Peroxidase
Toxin sensor
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Summary:An interference-based biosensor for amygdalin, a cyanogenic glycoside, is developed. Horseradish peroxidase (HRP) is adsorbed on a pyrolytic graphite (PG) electrode and then emulsin is cross-linked with glutaraldehyde on the electrode surface. The prepared bienzyme electrode is poised at −300 mV versus Ag/AgCl for 40 s to reduce dissolved O 2 to H 2O 2 at the PG surface. Then the potential is stepped to 0 mV, at which the accumulated H 2O 2 is reduced and the O 2 reduction does not proceed. Since the H 2O 2 reduction is catalyzed by HRP, the transient cathodic current is inhibited by cyanide, which is liberated from amygdalin by emulsin. Therefore, the transient current is a function of the amygdalin concentration. This sensor responds to 10 −6–10 −3 M amygdalin. Sensors for more concentrated amygdalin is prepared by using thinner emulsin films. This bienzyme electrode functions even in two-electrode mode.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(98)00150-6