In situ preparation of overoxidized PPy/ oPPD bilayer biosensors for the determination of glucose and cholesterol in serum

Overoxidized polypyrrole (PPy)/poly( o-phenylenediamine) ( oPPD) bilayer biosensors have been developed with the enzymes glucose oxidase or cholesterol oxidase entrapped within an inner PPy layer onto which a non-conducting oPPD film was then electrodeposited. Electrosynthesis of the polymer layers...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 1999-09, Vol.57 (1), p.219-226
Main Authors: Vidal, J.C, Garcı́a, E, Castillo, J.R
Format: Article
Language:English
Subjects:
Bilayer biosensor
Cholesterol determination
Enzyme entrapment
Flow system
Glucose determination
Overoxidized polypyrrole
Poly( o-phenylenediamine)
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Summary:Overoxidized polypyrrole (PPy)/poly( o-phenylenediamine) ( oPPD) bilayer biosensors have been developed with the enzymes glucose oxidase or cholesterol oxidase entrapped within an inner PPy layer onto which a non-conducting oPPD film was then electrodeposited. Electrosynthesis of the polymer layers was carried out in situ in a flow injection system with continuous circulation of a solution containing the monomer (plus the enzyme in the case of pyrrole). The analytical performance of this type of biosensor in the amperometric determination of glucose and cholesterol was compared with that of a PPy single layer sensor and their selectivity against electroactive substances frequently present in biological media was assessed by using them to determine both substrates in reference artificial serum.
ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(99)00082-9