Characterization of Actinobacillus actinomycetemcomitans leukotoxin pore formation in HL60 cells

The mechanism of cell death induced by Actinobacillus actinomycetemcomitans leukotoxin (LTX) has been investigated with flow cytometry and patch electrode recording using cultured HL60 cells. The kinetics of propidium iodide (PI) positive staining of HL60 cells was measured as a function of LTX conc...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1998-03, Vol.1406 (2), p.175-187
Main Authors: Karakelian, Darcy, Lear, James D, Lally, Edward T, Tanaka, Jacqueline C
Format: Article
Language:English
Subjects:
Actinobacillus actinomycetemcomitans (Pathology)
Actinobacillus actinomycetemcomitans leukotoxin
Aggregatibacter actinomycetemcomitans
Bacterial toxin
Bacterial Toxins - pharmacology
Cell Death
Cytotoxins - pharmacology
Electric Conductivity
Exotoxins - pharmacology
Flow Cytometry
HL60 cell
Humans
Ion channel
Ion Channels - metabolism
Kinetics
Membrane pore
Patch clamp
Patch-Clamp Techniques
RTX toxin
Single channel conductance
Tumor Cells, Cultured
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Summary:The mechanism of cell death induced by Actinobacillus actinomycetemcomitans leukotoxin (LTX) has been investigated with flow cytometry and patch electrode recording using cultured HL60 cells. The kinetics of propidium iodide (PI) positive staining of HL60 cells was measured as a function of LTX concentration at 37°C. Results showed a concentration-dependent decrease in the t k times. Cell kill was slow at
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/S0925-4439(98)00002-7