Glycoprotein composition in cyclophosphamide-induced lung fibrosis

The present study investigated the glycosylation state of proteins in lung tissue of a cyclophosphamide-induced model of pulmonary fibrosis in rats. In fibrotic lung, the carbohydrate constituents (total hexose, fucose, sialic acid and hexosamine) of salt-soluble, collagenase, elastase and papain di...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1998-08, Vol.1407 (2), p.125-134
Main Authors: Venkatesan, Narayanan, Punithavathi, Durairaj, Chandrakasan, Gowri
Format: Article
Language:English
Subjects:
Animals
Cyclophosphamide
Cyclophosphamide - pharmacology
Disease Models, Animal
Endopeptidases - metabolism
Fibrosis - chemically induced
Glycoprotein
Glycoprotein transferase
Glycoproteins - chemistry
Glycosyltransferases - analysis
Hydroxyproline - metabolism
Lung - enzymology
Lung - pathology
Lung fibrosis
Male
Monosaccharides - analysis
Rats
Rats, Wistar
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Summary:The present study investigated the glycosylation state of proteins in lung tissue of a cyclophosphamide-induced model of pulmonary fibrosis in rats. In fibrotic lung, the carbohydrate constituents (total hexose, fucose, sialic acid and hexosamine) of salt-soluble, collagenase, elastase and papain digested glycoproteins were significantly higher compared to normal lungs. Interestingly, fibrotic lung tissues had higher activities of mannosyl, glucosyl, galactosyl, sialyl and fucosyl transferases than normal lung tissues. Similarly, mannosyl, glucosyl, galactosyl, sialyl and fucosyl transferases were higher in serum from rats with fibrosis than in that from normals. These data indicate that glycoprotein metabolism is significantly altered from normal in animals with interstitial lung fibrosis.
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/S0925-4439(98)00035-0