Octopus rhodopsin photoreversibility of a crude extract from whole retina over several weeks' duration

A lipid/detergent micellar crude extract from whole octopus retinas when illuminated with blue light under slightly acidic conditions of pH 6·7 produces acid metarhodopsin that can be quantitatively converted to rhodopsin with red light. The octopus rhodopsin in detergent micelles was recycled at 25...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1998, Vol.13 (1), p.61-65
Main Authors: Ostrovsky, Michail A., Weetall, Howard H.
Format: Article
Language:English
Subjects:
octopus rhodopsin
photoreversibility
soluble cellular antioxidants
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Summary:A lipid/detergent micellar crude extract from whole octopus retinas when illuminated with blue light under slightly acidic conditions of pH 6·7 produces acid metarhodopsin that can be quantitatively converted to rhodopsin with red light. The octopus rhodopsin in detergent micelles was recycled at 25°C at least 50 times over a period of several weeks. This material has characteristics similar to some bacteriorhodopsin mutants. With improved stability and genetic modification, the invertebrate-retinal-containing protein may be a candidate for information storage and retrieval material.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(97)00078-X