Use of substrate coated electrodes and AC impedance spectroscopy for the detection of enzyme activity

A biosensor for collagenase detection was developed which detected the change in impedance caused by proteolytic digestion of gelatin coated interdigitated gold electrodes. The concentration of gelatin in the layer did not greatly affect the impedance measurements. Enzyme degradation of the layer pr...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1998-03, Vol.13 (5), p.511-518
Main Authors: Saum, A.G.E, Cumming, R.H, Rowell, F.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosensor
Biosensors
Biotechnology
collagenase
Fundamental and applied biological sciences. Psychology
gelatin
impedance
Methods. Procedures. Technologies
protease
Various methods and equipments
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Summary:A biosensor for collagenase detection was developed which detected the change in impedance caused by proteolytic digestion of gelatin coated interdigitated gold electrodes. The concentration of gelatin in the layer did not greatly affect the impedance measurements. Enzyme degradation of the layer produced a rapid rise in impedance when a critical thickness was reached. The change in impedance with protease digestion was correlated with solubilisation of the gelatin layer as measured by weight loss of the gelatin layer from the sensor surface. The response time of the device was greatly reduced by stirring the fluid around the biosensor. The ability to detect the gelatin coating on the sensor was severely impaired by the presence of electrolyte. The implications of these findings for further biosensor development are discussed.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(97)00129-2