Analysis of rat amylin amide from commercial sources: Identification of a mercury complex

Analysis of four commercial samples of rat amylin amide by HPLC, CZE and biological activity showed one sample (A) was different from the other three (B, C and D). Additional structural analysis of sample A (Edman sequencing, amino acid analysis, 1H NAMR and FAB-MS) suggested it was indeed rat amyli...

Full description

Saved in:
Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1991, Vol.1 (8), p.415-420
Main Authors: Cody, Wayne L., Giordani, Anne B., Werness, Stephen, Reily, Michael D., Bristol, James A., Zhu, Guochang, Dudley, David T.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Analysis of four commercial samples of rat amylin amide by HPLC, CZE and biological activity showed one sample (A) was different from the other three (B, C and D). Additional structural analysis of sample A (Edman sequencing, amino acid analysis, 1H NAMR and FAB-MS) suggested it was indeed rat amylin amide. However, electrospray ionization mass spectrometry and scanning electron microscopy showed sample A contained mercury covalently bound to the side chains of the cysteines in positions 2 and 7. Graphic
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(00)80268-1