A uniquely modified RNA: Introduction of a single RNA cleavage agent into the M1 ribozyme

We describe an efficient, four-step synthesis of an N 4 -modified cytidine phosphoramidite reagent ( 1) that permits nonnative functionality to be introduced into a synthetic oligoribonucleotide. This reagent was used to prepare a 377-nt E. coli M1 ribozyme equipped with a single RNA cleavage agent...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1994-09, Vol.4 (17), p.2133-2138
Main Authors: Richardson, Paul L., Gross, Margaret L., Light-Wahl, Karen J., Smith, Richard D., Schepartz, Alanna
Format: Article
Language:English
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Summary:We describe an efficient, four-step synthesis of an N 4 -modified cytidine phosphoramidite reagent ( 1) that permits nonnative functionality to be introduced into a synthetic oligoribonucleotide. This reagent was used to prepare a 377-nt E. coli M1 ribozyme equipped with a single RNA cleavage agent at nucleotide 11. We describe an efficient, four-step synthesis of an N 4 -modified cytidine phosphoramidite reagent ( 1) that permits nonnative functionality to be introduced into a synthetic oligoribonucleotide. This reagent was used to prepare a 377-nt E. coli M1 ribozyme equipped with a single RNA cleavage agent at nucleotide 11.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(01)80116-5