A synthetic glycopeptide of human myelin oligodendrocyte glycoprotein to detect antibody responses in multiple sclerosis and other neurological diseases

• Published in: Bioorganic & medicinal chemistry letters 1999-01, Vol.9 (2), p.167-172
• Main Authors: Mazzucco, Silvia, Matà, Sabrina, Vergelli, Marco, Fioresi, Rita, Nardi, Elena, Mazzanti, Benedetta, Chelli, Mario, Lolli, Francesco, Ginanneschi, Mauro, Pinto, Francesco, Massacesi, Luca, Papini, Anna Maria
• Format: Article
• Language: English
• Subjects: Amino Acids - chemistry; Amyotrophic Lateral Sclerosis - immunology; Antibodies; Antibodies - analysis; Biological and medical sciences; Encephalitis - immunology; Enzyme-Linked Immunosorbent Assay; Glycopeptides; Humans; Medical sciences; MOG; Multiple sclerosis; Multiple Sclerosis - immunology; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis; Mutagenesis; Myelin Proteins; Myelin-Associated Glycoprotein - blood; Myelin-Associated Glycoprotein - chemical synthesis; Myelin-Associated Glycoprotein - immunology; Myelin-Oligodendrocyte Glycoprotein; Myelitis - immunology; Neurology; Syndrome
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/S0960-894X(98)00698-2

Glycopeptides of hMOG(30–50) containing a glucosyl moiety on the side-chains of Asn, Ser or Hyp at position 31 were synthesised. Antibody titres to hMOG(30–50) and to its glucoderivatives were measured by ELISA in sera of patients affected by different neurological diseases. Anti-hMOG(30–50) antibodies were detected only using the glycopeptide [Asn 31( N-Glc)]hMOG(30–50). The glycopeptide [Asn 31( N-Glc)]hMOG(30–50) is the first specific antigen able to identify anti-hMOG(30–50) antibodies [hMOG(30–50), human myelin oligodendrocyte glycoprotein 30–50] in sera of patients affected by different neurological diseases. On the contrary, [Ser 31( O-Glc)]hMOG(30–50) and [Hyp 31( O-Glc)]hMOG(30–50) did not allow the antibody recognition.