Differentiation of Lactobacillus helveticus strains using molecular typing methods

Three molecular typing methods: SDS-PAGE, amplification of the 16S-23S internal transcribed spacer (ITS) loci by the polymerase chain reaction (PCR), and random amplification of polymorphic DNA (RAPD), were examined for their ability to type sixteen strains of Lactobadllus helveticus. Strains were g...

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Bibliographic Details
Published in:Food research international 1996, Vol.29 (5), p.451-455
Main Authors: Drake, M.A., Small, C.L., Spence, K.D., Swanson, B.G.
Format: Article
Language:English
Subjects:
16S rRNA
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
identification
lactic acid bacteria
Lactobacillus
Lactobacillus helveticus
Microbiology
PCR
RAPD
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Summary:Three molecular typing methods: SDS-PAGE, amplification of the 16S-23S internal transcribed spacer (ITS) loci by the polymerase chain reaction (PCR), and random amplification of polymorphic DNA (RAPD), were examined for their ability to type sixteen strains of Lactobadllus helveticus. Strains were grouped based on SDS-PAGE and ITS amplification patterns. All sixteen strains were distinguished by RAPD. Molecular typing methods allow rapid and relatively inexpensive ways to strain type L. helveticus and potentially other dairy lactobacilli.
ISSN:0963-9969
1873-7145
DOI:10.1016/S0963-9969(96)00059-2