Synthesis of an antisense oligonucleotide targeted against C-raf kinase: efficient oligonucleotide synthesis without chlorinated solvents

It is demonstrated that a solution of dichloroacetic acid in toluene removes dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothioate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on solid support cleanly and efficiently. It is therefore suggested to replac...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry 1999-03, Vol.7 (3), p.435-439
Main Authors: Krotz, A H, Cole, D L, Ravikumar, V T
Format: Article
Language:English
Subjects:
Base Sequence
Chlorine - chemistry
Chromatography, High Pressure Liquid
Oligonucleotides, Antisense - chemical synthesis
Oligonucleotides, Antisense - isolation & purification
Proto-Oncogene Proteins c-raf - genetics
Solvents - chemistry
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Summary:It is demonstrated that a solution of dichloroacetic acid in toluene removes dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothioate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on solid support cleanly and efficiently. It is therefore suggested to replace health hazardous dichloromethane which is typically used in oligonucleotide synthesis as solvent for DMTr-removal by toluene.
ISSN:0968-0896
DOI:10.1016/S0968-0896(98)00253-3