Development and evaluation of a kit formulation for the preparation of 99mTc-DMP-HSA, a new tracer agent for radionuclide ventriculography

• Published in: Nuclear medicine and biology 1997-08, Vol.24 (6), p.571-578
• Main Authors: Verbeke, Kristin A., Schiepers, Christiaan W., Wyndaele, Dirk N., Baetens, Jan A., Verbeke, Geert N., De Roo, Michel J., Verbruggen, Alfons M.
• Format: Article
• Language: English
• Subjects: 3-Dimercaptopropionyl-human serum albumin; 99mTc-DMP-HSA; Biological and medical sciences; Cardiovascular system; Humans; Investigative techniques, diagnostic techniques (general aspects); Kit formulation; Male; Medical sciences; Organotechnetium Compounds - chemistry; Organotechnetium Compounds - pharmacokinetics; Quality Control; Radiation Dosage; Radionuclide investigations; Radionuclide Ventriculography; Reagent Kits, Diagnostic; Sulfhydryl Compounds - chemistry; Sulfhydryl Compounds - pharmacokinetics; Technetium Tc 99m Aggregated Albumin - chemistry; Technetium Tc 99m Aggregated Albumin - pharmacokinetics; Technetium-99m; Technetium-99m-labeled red blood cells; Ventriculography
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/S0969-8051(97)00019-X

This study presents the development of a kit formulation for the preparation of 99mTc-DMP-HSA, followed by a comparison of such kit-prepared 99mTc-DMP-HSA to 99mTc-RBCs in a volunteer. Reconstitution of the labeling kits with up to 5.55 GBq 99mTc afforded 99mTc-DMP-HSA preparations with a >95% radiochemical purity for up to 8 h. Only minor differences were observed in the global distribution of both tracer agents, whereas the calculated ejection fractions were almost identical. The effective dose equivalent of 99mTc-DMP-HSA is 8.68 μSv/MBq.