Comparative investigations of the effects of X- and UV-irradiation on lysozyme in the absence or presence of additives

The radiation damage of lysozyme has been investigated in aqueous solution, after preceding X- and UV-irradiation in the absence or presence of various additives. Activity measurements and UV absorption and fluorescence spectroscopy turned out to be powerful screening techniques for disclosing the d...

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Bibliographic Details
Published in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2003-06, Vol.67 (3), p.479-486
Main Authors: Durchschlag, Helmut, Hefferle, Thomas, Zipper, Peter
Format: Article
Language:English
Subjects:
Additives
Lysozyme
Modeling
Radiation damage
Structure and function
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Summary:The radiation damage of lysozyme has been investigated in aqueous solution, after preceding X- and UV-irradiation in the absence or presence of various additives. Activity measurements and UV absorption and fluorescence spectroscopy turned out to be powerful screening techniques for disclosing the damages (inactivation, aggregation, loss of aromatics) and modifications (protection) provided by many additives. Advanced computer modeling approaches were used to visualize the protein atoms and the water molecules bound preferentially on the protein surface. Correlations between the position of radiosensitive amino acid residues and occurring radiation damages are discussed.
ISSN:0969-806X
1879-0895
DOI:10.1016/S0969-806X(03)00089-6