Differences in the composition of phenolics and fatty acids of cultured mycobiont and thallus of Physconia distorta

Germinated ascospores of Physconia distorta on an inorganic medium were grown on an enriched medium (Lilly–Barnet medium with 4% glucose), on which they produced swollen hyphae which included numerous lipid droplets. Secondary metabolites extracted from the cultured fungus were analyzed by high perf...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2003-02, Vol.41 (2), p.175-180
Main Authors: Molina, M.C., Crespo, A., Vicente, C., Elix, J. A.
Format: Article
Language:English
Subjects:
ACIDE GRAS
ACIDOS GRASOS
Axenic culture
Biological and medical sciences
CHAMPIGNON
CHEMICAL COMPOSITION
Chemical constitution
COMPOSE PHENOLIQUE
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
COMPUESTOS FENOLICOS
CULTIVO IN VITRO
CULTURE IN VITRO
Fatty acid
FATTY ACIDS
Fundamental and applied biological sciences. Psychology
FUNGI
HONGOS
IN VITRO CULTURE
LICHEN
LICHENES
LIQUENES
METABOLITE
METABOLITES
METABOLITOS
Phenolic acid
PHENOLIC COMPOUNDS
Physconia distorta
Plant physiology and development
SIMBIONTES
SYMBIONTS
SYMBIOTE
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Summary:Germinated ascospores of Physconia distorta on an inorganic medium were grown on an enriched medium (Lilly–Barnet medium with 4% glucose), on which they produced swollen hyphae which included numerous lipid droplets. Secondary metabolites extracted from the cultured fungus were analyzed by high performance liquid chromatography and mass spectrometry. Analyses of superficial compounds established the presence of stearic, linoleic and oleic acids, and the corresponding triglycerides. Malonprotocetraric acid, several unknown pigments, and a series of fatty acids were detected as internal compounds. The qualitative and quantitative phenolic and fatty acid composition from the isolated fungal partner and lichenized thalli were different. Fatty acids were the main substances found by liquid chromatography in extracts of axenic cultures, while extracts from intact thalli contain phenolic acids as the main compounds. These results can be explained by metabolic differences as a result of the different available nutrients.
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(02)00017-7