Identification of stathmin-like proteins in plants

Stathmin-like proteins were identified in broad bean, green bean, pea, soybean, and cowpea extracts by western blotting after SDS PAGE and native isoelectric focusing (IEF). The subunit molecular mass of these proteins ranged from 14–23 kDa in leaf, root and stem tissues, but higher molecular mass f...

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Bibliographic Details
Published in:Plant physiology and biochemistry 1998-06, Vol.36 (6), p.449-455
Main Authors: Flurkey, William H., Kelley, Marc, Hughes, James P., Mulkey, Timothy, Prentice, David A.
Format: Article
Language:English
Subjects:
5-bromo-4-chloro-3-indoyl-phosphate
Agronomy. Soil science and plant productions
BCIP
Biological and medical sciences
CITOPLASMA
CYTOPLASM
CYTOPLASME
Economic plant physiology
Fundamental and applied biological sciences. Psychology
GLYCINE MAX
IDENTIFICACION
IDENTIFICATION
IEF
ISOELECTRIC POINT
Metabolism
Metabolism. Physicochemical requirements
molecular mass
MOLECULAR WEIGHT
NBT
nitro blue tetrazolium chloride
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
PESO MOLECULAR
PHASEOLUS VULGARIS
PISUM SATIVUM
Plant physiology and development
plante
POIDS MOLECULAIRE
POINT ISOELECTRIQUE
polyvinylidene difluoride
PROTEINAS
PROTEINE
PROTEINS
PUNTO ISOELECTRICO
PVDF
Stathmin
tissues
VICIA FABA
VIGNA RADIATA
VIGNA UNGUICULATA
ZEA MAYS
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Summary:Stathmin-like proteins were identified in broad bean, green bean, pea, soybean, and cowpea extracts by western blotting after SDS PAGE and native isoelectric focusing (IEF). The subunit molecular mass of these proteins ranged from 14–23 kDa in leaf, root and stem tissues, but higher molecular mass forms were observed in germinated seed (cotyledon) extracts. After IEF, multiple forms of stathmin-like proteins were observed in different tissues from all plant extracts. In general, the pIs ranged from 3.5 to 5.0, although each tissue showed a unique IEF pattern of stathmin-like proteins. Stathmin-like proteins in plants appear to be similar to mammalian stathmin in size, pI, and immunological determinants.
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(98)80209-X