A simple method for purification of genomic DNA from whole blood using Fe3O4/Au composite particles as a carrier

• Published in: Journal of Medical Colleges of PLA 2009-08, Vol.24 (4), p.239-243
• Main Authors: Ming, Zhao, Xianqing, Zhang, Sen, Wang, Chao, Chen, Yali, Cui
• Format: Article
• Language: English
• Subjects: DNA纯化; PCR扩增; 基因组DNA; 复合粒子; 氯化钠浓度; 琼脂糖凝胶电泳
• ISSN: 1000-1948
• DOI: 10.1016/S1000-1948(09)60044-7

Objective： To establish a method of genomic DNA extraction from whole blood using Fe3O4/Au composite particles as a carrier. Methods： Two crucial conditions （sodium chloride concentration and amount of the magnetic particles） were optimized and 8 different human whole blood samples were used to purify genomic DNA under the optimal condition. Then agarose gel electrophoresis and polymerase cbain reaction （PCR） were performed. Results： The optimal binding condition was 1.5 mol/L NaC1/10% PEG, and the optimal amount of Fe3O4/Au composite particles was 600μg. The yields of the genomic DNA from 100μl of different whole blood samples were 2-5 μg, and the ratio of A260/A280 was in the range of 1.70-1.90. The size of genomic DNA was about 23 kb and the PCR was valid. Conclusion： The purification system using Fe3O4/Au composite microparticles has advantages in high yield, high purity, ease of operating, time saving and avoiding centrifugation. The purified sample was found to function satisfactorily in PCR amplification.