Engineering chloroperoxidase for activity and stability

Random mutagenesis of Caldariomyces fumago was carried out to produce a chloroperoxidase (CPO) with enhanced activity in mixtures of aqueous buffers and organic cosolvents. A mutant CPO with a 3.4-fold increased activity in 40% aqueous tert-butyl alcohol was obtained. A CPO-surfactant conjugate, pre...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Enzymatic, 2001-01, Vol.11 (4-6), p.765-769
Main Authors: van de Velde, F., Bakker, M., van Rantwijk, F., Rai, G.P., Hager, L.P., Sheldon, R.A.
Format: Article
Language:English
Subjects:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Chloroperoxidase
Fundamental and applied biological sciences. Psychology
Immobilisation
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Organic solvents
Random mutagenesis
Sulfoxidation
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Summary:Random mutagenesis of Caldariomyces fumago was carried out to produce a chloroperoxidase (CPO) with enhanced activity in mixtures of aqueous buffers and organic cosolvents. A mutant CPO with a 3.4-fold increased activity in 40% aqueous tert-butyl alcohol was obtained. A CPO-surfactant conjugate, prepared by colyophilisation, mediated the oxidation of thioanisole to its (R)-sulfoxide by TBHP in water-saturated isooctane. Copolymerisation of CPO and a toluenediisocyanate prepolymer resulted in a stable preparation that mediated the oxidation of indole to 2-oxindole in a range of organic solvents. The highest yield was obtained in 1-octanol.
ISSN:1381-1177
1873-3158
DOI:10.1016/S1381-1177(00)00022-9