Remarkable decrease in d-amino acid oxidase activity in the kidney of both prone and resistant strains of senescence-accelerated mouse

d-Amino acid oxidase (DAO) activity was remarkably low in the kidney of both prone P1 and resistant R1 strains of the senescence-accelerated mouse (SAM) compared with that of AKR/J mouse, in spite of the presence of a nearly equal amount of protein as demonstrated by Western blot analysis. A nucleot...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Enzymatic, 2001-02, Vol.12 (1), p.115-119
Main Authors: Yokoyama, Shin-ichiro, Ohishi, Nobuko, Nagai, Miyuki, Komura, Sadaaki, Hosokawa, Masanori, Yagi, Kunio
Format: Article
Language:English
Subjects:
d-Amino acid oxidase
Point mutation
Senescence-accelerated mouse
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Summary:d-Amino acid oxidase (DAO) activity was remarkably low in the kidney of both prone P1 and resistant R1 strains of the senescence-accelerated mouse (SAM) compared with that of AKR/J mouse, in spite of the presence of a nearly equal amount of protein as demonstrated by Western blot analysis. A nucleotide sequence analysis of DAO-cDNA from SAMP1/Yag kidney indicated that the 541st guanine was replaced by adenine, resulting in the change of the 181st amino acid from glycine to arginine. The results of reverse transcription-polymerase chain reaction-restriction fragment length polymorphism analysis indicate the presence of this mutation in all SAM strains examined (P1, P2, P3 and R5).
ISSN:1381-1177
1873-3158
DOI:10.1016/S1381-1177(00)00211-3