Coke oven workers study: the effect of exposure and GSTM1 and NAT2 genotypes on DNA adduct levels in white blood cells and lymphocytes as determined by [formula omitted]-postlabelling

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis 1998-08, Vol.416 (1), p.67-84
• Main Authors: Binková, Blanka, Topinka, Jan, Mračková, Gabriela, Gajdošová, Dagmar, Vidová, Paulı́na, Stávková, Zdena, Peterka, Vı́t, Pilčı́k, Tomáš, Rimár, Vladimı́r, Dobiáš, Lubomı́r, Farmer, Peter B., Šrám, Radim J.
• Format: Article
• Language: English
• Subjects: Coke oven worker; DNA adduct by [formula omitted]-postlabelling; GSTM1 and NAT2; Personal monitoring of PAH; Vitamin A; Vitamin E; WBC and lymphocyte
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/S1383-5718(98)00061-8

The DNA adduct levels in total white blood cells (WBC) and lymphocytes (LYM) isolated from the blood of the same individuals were evaluated using the 32 P -postlabelling assay for bulky aromatic adducts. In this study, 68 male coke oven workers and 56 machine workers as a matched control were enrolled. Personal monitors were used to evaluate exposure to eight carcinogenic PAHs, including B[ a]P, during an 8-h working shift. The exposure among coke oven workers ranged widely from 0.6 to 547 μg/m 3 and from 2 to 62,107 ng/m 3, for carcinogenic PAHs and B[ a]P, respectively. The respective values in controls were from 0.07–1.64 μg/m 3 and from 1–63 ng/m 3. A significant correlation between WBC– and LYM–DNA adduct levels was found ( r=0.591, P