Sample preparation protocols for MALDI-MS of peptides and oligonucleotides using prestructured sample supports

This contribution provides a set of protocols for MALDI-MS sample preparation of peptides and oligonucleotides on prestructured sample supports. The protocols have been optimized for high detection sensitivity, robust performance, ease of use, and include sample purification and concentration. Some...

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Bibliographic Details
Published in:International journal of mass spectrometry 2003-03, Vol.226 (1), p.163-180
Main Authors: Nordhoff, Eckhard, Schürenberg, Martin, Thiele, Gabriela, Lübbert, Christine, Kloeppel, Klaus-Dieter, Theiss, Dorothea, Lehrach, Hans, Gobom, Johan
Format: Article
Language:English
Subjects:
MALDI
Oligonucleotide
Peptide
Proteomics
Sample preparation
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Summary:This contribution provides a set of protocols for MALDI-MS sample preparation of peptides and oligonucleotides on prestructured sample supports. The protocols have been optimized for high detection sensitivity, robust performance, ease of use, and include sample purification and concentration. Some protocols were optimized for manual preparation of individual samples. Others were developed for the use of automated pipetting stations and optimized for high throughput protein identification and DNA sequence analysis.
ISSN:1387-3806
1873-2798
DOI:10.1016/S1387-3806(02)00978-8