The effect of liquid chromatography eluents and additives on the positive ion responses of cocaine, benzoylecgonine, and ecgonine methyl ester using electrospray ionization

We investigated the effect of common chromatography eluents and additives on the positive ion responses of ecgonine methyl ester (EME), benzoylecgonine (BZE), and cocaine (COC) using electrospray ionization (ESI). Primarily [M+H] + ions were observed, although decomposition of EME and COC to ecgonid...

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Bibliographic Details
Published in:International journal of mass spectrometry 2003-06, Vol.227 (2), p.247-258
Main Authors: Jeanville, Patrick M., Estapé, Estela S., de Jeanville, Ivette Torres-Negrón
Format: Article
Language:English
Subjects:
API/MS
Benzoylecgonine
Cocaine
Ecgonine methyl ester
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Summary:We investigated the effect of common chromatography eluents and additives on the positive ion responses of ecgonine methyl ester (EME), benzoylecgonine (BZE), and cocaine (COC) using electrospray ionization (ESI). Primarily [M+H] + ions were observed, although decomposition of EME and COC to ecgonidine methyl ester gave a sizable peak at m/ z 182.0. The results showed that the sensitivity for the test analytes was greatest in a mobile-phase consisting of a 1:1 mixture of 60% acetonitrile/40% acetone:100 mM ammonium acetate. There was no evidence of a correlation between sensitivity of [M+H] + ions and solution pH. Adducts derived from addition of ammonium salts and ammonium hydroxide, along with cluster ions were not observed, although cationization did occur for BZE (
ISSN:1387-3806
1873-2798
DOI:10.1016/S1387-3806(03)00142-8