Non-invasive electrochemical hand-held biosensor as diagnostic indicator of dental diseases

• Published in: Electrochemistry communications 2003-03, Vol.5 (3), p.225-229
• Main Authors: Ivnitski, Dmitri, Sitdikov, Ravil, Ivnitski, Natalia
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biosensors; Biotechnology; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Fundamental and applied biological sciences. Psychology; Hand-held amperometric biosensor; Medical sciences; Methods. Procedures. Technologies; Non tumoral diseases; Otorhinolaryngology. Stomatology; Periodontal diseases; Salivary peroxidase; Various methods and equipments
• ISSN: 1388-2481; 1873-1902
• DOI: 10.1016/S1388-2481(03)00023-7

A new hand-held amperometric biosensor for assay of salivary peroxidase in human secretions is described. The biosensor comprises a flow through amperometric detector coupled with a micropipette, and electronics (an amplifier, peak detector, microprocessor, and display). A micropipette, amplifier, peak detector, and microprocessor have been built in-house. All operations, such as sample injection, electrochemical detection and data processing are accomplished using only one action, by pressing the plunger of the micropipette. Because the use of a peristaltic pump is completely eliminated, the consumption of both samples and reagents is significantly reduced. The biosensor has been tested as a diagnostic indicator of dental diseases. It is shown that there is a functional relationship between the total level of salivary peroxidase and the clinical status of the periodontal disease. By using commercial available kits with peroxidase-labeled conjugates, the biosensor has an excellent potential for detection of different hormones, drugs, viruses, antibodies, and bacteria.