Bilirubin Biosensing by Using of Catalase and ZnS Nanoparticles as Modifier

To find a stable bilirubin biosensor we used of catalase and Zns nanoparticles (Nps); the detection of bilirubin in this research designed on Bioelectrochemical measurement cyclic voltammetry (CV) techniques. ZnS Nanoparticles synthesized and investigated by X-Ray diffraction (XRD), UV-Visible (UV-V...

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Bibliographic Details
Published in:International journal of electrochemical science 2016, Vol.11 (3), p.2029-2045
Main Authors: Dehghani, Hamideh, Khoramnejadian, Shahrzad, Mahboubi, Mohammad, Sasani, Masoomeh, Ghobadzadeh, Samira, Haghighi, Saghar Mowlazadeh, Negahdary, Masoud
Format: Article
Language:English
Subjects:
Bilirubin
Biosensing
Catalase
Zns nanoparticles
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Summary:To find a stable bilirubin biosensor we used of catalase and Zns nanoparticles (Nps); the detection of bilirubin in this research designed on Bioelectrochemical measurement cyclic voltammetry (CV) techniques. ZnS Nanoparticles synthesized and investigated by X-Ray diffraction (XRD), UV-Visible (UV-VIS) and scanning electron microscopy (SEM). The redox peaks studied in the range of +0.5 to -0.5 V and formal potential (E°) calculated as 40 mv; also ΔEp =70 mV. In this research the anodic peak current of showed a linear relationship for different concentrations of bilirubin in the range of 3 to 50 μM. The linear equation was expressed as ipa (μA) = -0.1417-0.3661 (μM) (R2= 0.9885) with a detection limit of 2 μM for this research. The optimum pH effect was 7.0 and maximum temperature response was around 25 °C. Interference agents not showed a significant effect on this bilirubin biosensor.
ISSN:1452-3981
1452-3981
DOI:10.1016/S1452-3981(23)16080-9