A simple method for immunodetection of membrane-associated proteins

The main objective of the present study was to identify membrane antigenic proteins using immunodiffusion and Western blotting techniques. This multidisciplinary experiment integrates biochemical principles with immunological and microbiological concepts. Pasteurella multocida, the causative agent o...

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Bibliographic Details
Published in:Biochemistry and molecular biology education 2000-09, Vol.28 (5), p.256-260
Main Authors: Daghastanli, Katia R.P., Ferreira, Rinaldo B., Thedei, Geraldo, Ciancaglini, Pietro
Format: Article
Language:English
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Summary:The main objective of the present study was to identify membrane antigenic proteins using immunodiffusion and Western blotting techniques. This multidisciplinary experiment integrates biochemical principles with immunological and microbiological concepts. Pasteurella multocida, the causative agent of fowl cholera and other diseases like haemorrhagic septicaemia in many wild and domestic animals, was chosen as a biological model in order to obtain membrane antigenic proteins. We designed some simple laboratory experiments: partial purification of IgG, study of the effect of detergent on immunodiffusion, electrophoresis and Western blotting of membrane protein fraction of Pasteurella multocida. The results obtained with these techniques revealed that the membrane antigenic proteins Pasteurella multocida presented had Mr's of around 110, 104, 86, 71, 65 and 40kDa.
ISSN:1470-8175
1539-3429
DOI:10.1016/S1470-8175(00)00022-9