P2X2 receptor expression by interstitial cells of Cajal in vas deferens implicated in semen emission

• Published in: Autonomic neuroscience 2000-11, Vol.84 (3), p.147-161
• Main Authors: BURTON, Lucille D, HOUSLEY, Gary D, SALIH, Salam G, JÄRLEBARK, Leif, CHRISTIE, David L, GREENWOOD, Denise
• Format: Article
• Language: English
• Subjects: Acridine Orange; Adenosine Triphosphate - physiology; Afferent Pathways - anatomy & histology; Afferent Pathways - physiology; Alternative Splicing; Animals; Biological and medical sciences; Biomarkers; Coloring Agents; Ejaculation - physiology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Guinea Pigs; Immunoenzyme Techniques; In Situ Hybridization; Male; Mammalian male genital system; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Morphology. Physiology; Muscle Contraction; Muscle, Smooth - innervation; Muscle, Smooth - physiology; Phosphopyruvate Hydratase - analysis; Protein Subunits; Rats; Rats, Wistar; Receptors, Purinergic P2 - biosynthesis; Receptors, Purinergic P2 - genetics; Receptors, Purinergic P2X2; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Species Specificity; Staining and Labeling; Sympathetic Nervous System - anatomy & histology; Sympathetic Nervous System - physiology; Tolonium Chloride; Vas Deferens - cytology; Vas Deferens - metabolism; Vertebrates: reproduction
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/s1566-0702(00)00200-9

Male reproduction is dependent upon seminal emission mediated by vas deferens contraction. This drives spermatic fluid to the prostatic urethra during ejaculation. We localize interstitial cells of Cajal (ICC), which express P2X2 receptor, subunits of ATP-gated ion channels, to rat, mouse and guinea-pig vas deferens submucosa. Reverse transcription-polymerase chain reaction (RT-PCR) analysis of rat vas deferens resolved two functional splice variant transcripts of the P2X2 receptor subunit. The P2X2 receptor mRNA was localized principally within the lamina propria (submucosal) region of the rat vas deferens using in situ hybridization (ISH) and in situ RT-PCR-ISH. Immunohistochemistry using rat, mouse and guinea-pig vas deferens tissues confirmed expression of P2X2 receptor protein within the lamina propria, particularly within a dense column of small spindle-shaped cells adjacent to the columnar epithelial cells which line the lumen. This immunoreactivity was co-localized with neurone-specific enolase (NSE) and c-Kit protein expression, gene markers for ICC. Mucosal mast cells were distinguished from ICC by toluidine blue staining. Choline acetyltransferase immunoreactivity, a marker for post-ganglionic parasympathetic innervation, occurred on the lateral margin of the lamina propria and extended into the inner longitudinal muscle layer. P2X1 receptor immunolabelling was associated with sympathetic innervation of the smooth muscle in the outer longitudinal and circular muscle layers, but not the inner longitudinal layer. The physiological significance of the vas deferens ICC which express P2X2 receptors remains to be established. Possible roles include regulation of smooth muscle activity or mucosal secretion utilizing local ATP signaling, both of which would affect semen transport.