ePS06.2 Immune cell localization in different compartments of human end-stage CF lungs

Objectives The localization of immune cells along airways, blood vessels or in parenchyma in CF lung disease has insufficiently been established. A comprehensive quantitative immunohistochemical analysis was performed on lung tissue from CF patients and healthy controls (HC). Methods Sections (8 mm)...

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Published in:Journal of cystic fibrosis 2015-06, Vol.14, p.S53-S53
Main Authors: Lammertyn, E.J, Vandermeulen, E, Bellon, H, Goeminne, P.C, Vanaudenaerde, B.M, Dupont, L.J
Format: Article
Language:English
Subjects:
Pulmonary/Respiratory
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Summary:Objectives The localization of immune cells along airways, blood vessels or in parenchyma in CF lung disease has insufficiently been established. A comprehensive quantitative immunohistochemical analysis was performed on lung tissue from CF patients and healthy controls (HC). Methods Sections (8 mm) of lung biopsies of CF patients taken during transplantation (n = 20) and control lung biopsies (n = 22) were stained for Th-lymphocytes (CD4), mast cells (tryptase) and eosinophils (EG-2). Quantification was performed by counting all single cells normalized over total area (mm2 ) of biopsy (Th cells), or in 10 high power fields averaged per compartment (airways, blood vessels and parenchyma) per subject (mast cells and eosinophils). Results CD4+ T-lymphocyte counts were significantly increased in CF vs. HC (p = 0.001) and were present as dispersed single cells (CF & HC), or organized in follicles mainly situated in parenchyma (predominantly CF). Both total & compartmental mast cell counts were significantly increased in CF vs. HC (all p
ISSN:1569-1993
1873-5010
DOI:10.1016/S1569-1993(15)30170-3