Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography-ultraviolet detection analysis

Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries we...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-10, Vol.810 (2), p.305-311
Main Authors: MATEO, Rafael, CASTELLS, Gloria, GREEN, Andy J, GODOY, Carlo, CRISTOFOL, Caries
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Bile - chemistry
Biliverdine - analysis
Biological and medical sciences
Birds - metabolism
Calibration
Chromatography, High Pressure Liquid
Ducks
Environmental Monitoring
Feces - chemistry
Fundamental and applied biological sciences. Psychology
Geese
General pharmacology
Indicators and Reagents
Lead Poisoning - metabolism
Medical sciences
Pharmacology. Drug treatments
Porphyrins - analysis
Raptors
Reproducibility of Results
Specimen Handling
Spectrophotometry, Ultraviolet
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Summary:Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(04)00663-4