The effect of horse serum on in vitro development of porcine parthenogenetic embryos

SUMMARY The objective of this study was to examine the effect of different sera and serum-like substances on the preimplantation development of porcine parthenogenetic embryos. Chemically activated (calcium ionophore A23187+cytochalasin B) pig oocytes were pre-cultured for five days. On day 5, the p...

Full description

Saved in:
Bibliographic Details
Published in:Reproductive biology 2012-03, Vol.12 (1), p.25-39
Main Authors: Hwang, In-Sul, Kim, Sung Woo, Byun, Sung June, Kim, Kyung-Woon, Chung, Hee Kyoung, Park, Jong-Ju, Gobianand, Kuppannan, Park, Jin-Ki, Yoo, Jae Gyu
Format: Article
Language:English
Subjects:
blastocyst apoptosis
blastocyst hatching
Obstetrics and Gynecology
parthenotes
pig
preimplantation development
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SUMMARY The objective of this study was to examine the effect of different sera and serum-like substances on the preimplantation development of porcine parthenogenetic embryos. Chemically activated (calcium ionophore A23187+cytochalasin B) pig oocytes were pre-cultured for five days. On day 5, the parthenogenetic embryos were treated with porcine follicular fluid (PFF), fetal bovine serum (FBS), horse serum (HS) or porcine serum albumin (PSA), and were cultured two more days. Horse serum was found to be the most effective protein source in enhancing parthenote development judging by blastocyst formation and hatching. Next, three different concentrations of HS (10, 20 and 30%) were used to determine the optimal HS concentration needed to improve the development of porcine parthenogenetic embryos. All HS concentrations increased the blastocyst cell number and decreased the incidence of blastocyst apoptotic cells with 20% being the most effective. In conclusion, horse serum enhanced parthenogenetic embryo development and the quality of porcine parthenogenetic embryos.
ISSN:1642-431X
DOI:10.1016/S1642-431X(12)60075-8