Relationship Between Heart Damages and HSPs mRNA in Persistent Heat Stressed Broilers

The relationship between myocardial cell damages and HSPs mRNA transcription in heat stressed broilers was studied using a spectrophotometer, the histopathological technique, and fluorescence quantitative reverse transcription PCR （FQ RT-PCR）. The results showed that the activities of creatine kinas...

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Bibliographic Details
Published in:Agricultural sciences in China 2007-02, Vol.6 (2), p.227-233
Main Author: SUN Pei-ming LIU Yu-tian ZHAO Yong-gang BAO En-dong WANG Zhi-liang
Format: Article
Language:English
Subjects:
FQRT-PCR
持续热应激
热休克蛋白
热损伤
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Summary:The relationship between myocardial cell damages and HSPs mRNA transcription in heat stressed broilers was studied using a spectrophotometer, the histopathological technique, and fluorescence quantitative reverse transcription PCR （FQ RT-PCR）. The results showed that the activities of creatine kinase （CK） and glutamic-pyruvic transaminase （GPT） were induction during the persistent heat stress. The major lesions of the myocardial fibers were granular degeneration and necrosis. The transcription of constitutive or cognate heat shock protein 70 （HSC70） mRNA was changeable. The transcription of heat shock protein 70 （HSPT0） mRNA was increased obviously in the course of persistent heat stress. The results showed that the change of HSC70 mRNA transcription was contrary to the activity of CK, and the level of HSC70 mRNA transcription must be used as a symbol of the myocardial cell damages in the course of persistent heat stress.
ISSN:1671-2927
DOI:10.1016/S1671-2927(07)60039-X